Hilton M.J. (Ed.) Skeletal Development and Repair. Methods and Protocols

Humana Press, 2014 — 328p. — (Methods in Molecular Biology 1130) — ISBN: 978-1-62703-989-5 (eBook), 978-1-62703-988-8 (Hardcover).Skeletal Development and Repair: Methods and Protocols is a compilation of a variety of skeletal research protocols utilizing the laboratory mouse as the platform for surgical manipulation and/or transplantation as well as the source of tissues and cells for in vitro culture and analyses. Chapters are written by experts in the field and cover topics including surgical, transplantation and organ culture methods that permit analyses of skeletal tissues undergoing repair in vivo and permits analyses of cellular interactions ex vivo, histological and molecular techniques developed to study gene and protein expression in whole embryos, skeletal tissues and tissue sections and in vitro primary cell culture protocols designed to assay gene function in specific cell populations. Written in the successful Methods in Molecular Biology series format chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and notes on troubleshooting and avoiding known pitfalls.
Authoritative and easily accessible, Skeletal Development and Repair: Methods and Protocols is a comprehensive laboratory manual for all levels of basic research scientists working in the broad fields of skeletal development and skeletal repair research.Overview of Skeletal Development
Overview of Skeletal Repair (Fracture Healing and Its Assessment)
Generation of Closed Transverse Fractures in Small Animals
The Murine Femoral Bone Graft Model and a Semiautomated Histomorphometric Analysis Tool
Surgical Induction of Posttraumatic Osteoarthritis in the Mouse
A Mouse Model of Flexor Tendon Repair
Cartilage Explant Cultures
Renal Capsule Transplantations to Assay Skeletal Angiogenesis
Whole-Mount Skeletal Staining
Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens
Preparation of Thin Frozen Sections from Nonfixed and Undecalcified Hard Tissues Using Kawamot’s Film Method (2012)
Demineralized Murine Skeletal Histology
Beta-Galactosidase Staining in the Skeleton
Whole-Mount In Situ Hybridization on Murine Skeletogenic Tissues
Nonradioactive In Situ Hybridization on Skeletal Tissue Sections
Radioactive In Situ Hybridization to Detect Gene Expression in Skeletal Tissue Sections
Proliferation Assays (BrdU and EdU) on Skeletal Tissue Sections
TUNEL Assay on Skeletal Tissue Sections to Detect Cell Death
Analysis of Chondrogenesis Using Micromass Cultures of Limb Mesenchyme
Isolation and Culture of Murine Primary Chondrocytes