Bugert P. (Ed.) Molecular Typing of Blood Cell Antigens

Humana Press, 2015 — 261p. — (Methods in Molecular Biology 1310) — ISBN: 978-1-4939-2690-9 (eBook), 978-1-4939-2689-3 (Hardcover).This volume aims to bring together a variety of protocols useful for DNA-based typing of blood cell antigens. Protocols range from simple approaches with low technical complexity to highly sophisticated modern developments. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Molecular Typing of Blood Cell Antigens summarizes contributions from leading scientist in the field DNA typing for blood cell antigens.Validation of Genotyping Protocols for Diagnostic Use
High-Resolution Melting Analysis of Single Nucleotide Polymorphisms
High-Speed Droplet-Allele-Specific Polymerase Chain Reaction for Genotyping of Single Nucleotide Polymorphisms
Blood Grouping Based on PCR Methods and Agarose Gel Electrophoresis
Parallel Donor Genotyping for 46 Selected Blood Group and 4 Human Platelet Antigens Using High-Throughput MALDI-TOF Mass Spectrometry
PCR with Sequence-Specific Primers for Typing of Diallelic Blood Groups
High-Resolution Melting Analysis for Genotyping Duffy Blood Group Antigens
Molecular RHD-RHCE Analysis by Multiplex PCR of Short Fluorescent Fragments
Microarrays in Blood Group Genotyping
Next-Generation Sequencing for Antenatal Prediction of KEL1 Blood Group Status
454-Sequencing for the KEL, JR, and LAN Blood Groups
Noninvasive Prenatal Blood Group Genotyping
Genotyping of Human Platelet Antigens by BeadChip Microarray Technology
Sequence-Based Typing for Platelet alloantigens
Miniaturized Technology for DNA Typing: Cassette PCR
Geno- and Phenotyping of Human Neutrophil Antigens
Allelic Discrimination by TaqMan-PCR for Genotyping of Human Neutrophil Antigens
Novel Approaches and Technologies in Molecular HLA Typing
Luminex-Based Methods in High-Resolution HLA Typing
In Silico HLA Typing Using Standard RNA-Seq Sequence Reads