Kurien B.T., Scofield R.H. (Eds.) Western Blotting. Methods and Protocols

Humana Press, 2015 — 509p. — (Methods in Molecular Biology 1312) — ISBN: 978-1-4939-2694-7 (eBook), 978-1-4939-2693-0 (Hardcover).This volume covers past and present western blot techniques, such as diffusion blotting, slice blotting, blotting of high and low molecular weight proteins, single cell blotting and automated blotting. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Western Blotting: Methods and Protocols will serve as an invaluable reference for those interested in further study into this fascinating field.The Early Days of Blotting
Origins of Protein Blotting
Western Blotting: Remembrance of Things Past
Simian Virus 40 and Protein Transfer
Western Blotting: An Introduction
From Little Helpers to Automation
Spectrophotometric Methods to Determine Protein Concentration
Solubilization of Proteins: The Importance of Lysis Buffer Choice
Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis
Diffusion Blotting: A Rapid and Simple Method for Production of Multiple Blots from a Single Gel
Multiple Immunoblots by Passive Diffusion of Proteins from a Single SDS-PAGE Gel
Slice Blotting
Localizing Proteins by Tissue Printing
Dot-Immunobinding Assay (Dot-Iba)
Analysis of Antibody Clonotype by Affinity Immunoblotting
Glycosaminoglycan Blotting and Detection After Electrophoresis Separation
A Well-Based Reverse-Phase Protein Array of Formalin-Fixed Paraffin-Embedded Tissue
Quantitative Computerized Western Blotting in Detail
Cationic Electrophoresis and Eastern Blotting
A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies