Castilho A. (Ed.) Glyco-Engineering. Methods and Protocols

Humana Press, 2015 — 439p. — (Methods in Molecular Biology 1321) — ISBN: 978-1-4939-2760-9 (eBook), 978-1-4939-2759-3 (Hardcover).Conceived with the intention of providing an array of strategies and technologies currently in use for glyco-engineering distinct living organisms, this book contains a wide range of methods being developed to control the composition of carbohydrates and the properties of proteins through manipulations on the production host rather than in the protein itself. The first five sections deal with host-specific glyco-engineering and contain chapters that provide protocols for modifications of the glycosylation pathway in bacteria, yeast, insect, plants and mammalian cells, while the last two sections explore alternative approaches to host glyco-engineering and selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and extensive, Glyco-Engineering: Methods and Protocols offers vast options to help researchers to choose the expression system and approach that best suits their intended protein research or applications.Current Approaches to Engineering N-Linked Protein Glycosylation in Bacteria
Inverse Metabolic Engineering for Enhanced Glycoprotein Production in Escherichia coli
GlycoSNAP: A High-Throughput Screening Methodology for Engineering Designer Glycosylation Enzymes
Production of Glycoproteins with Asparagine-Linked N-Acetylglucosamine in Escherichia coli
Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in E. coli
Progress in Yeast Glycosylation Engineering
Protein Production with a Pichia pastoris OCH1 Knockout Strain in Fed-Batch Mode
Engineering the Pichia pastoris N-Glycosylation Pathway Using the GlycoSwitch Technology
Development of a Valuable Yeast Strain Using a Novel Mutagenesis Technique for the Effective Production of Therapeutic Glycoproteins
An Overview and History of Glyco-Engineering in Insect Expression Systems
SweetBac: Applying MultiBac Technology Towards Flexible Modification of Insect Cell Glycosylation
Engineering Sialic Acid Synthesis Ability in Insect Cells
Engineering N-Glycosylation Pathway in Insect Cells: Suppression of β-N-Acetylglucosaminidase and Expression of β-1,4-Galactosyltransferase
N-Glyco-Engineering in Plants: Update on Strategies and Major Achievements
Gene Targeting for Precision Glyco-Engineering: Production of Biopharmaceuticals Devoid of Plant-Typical Glycosylation in Moss Bioreactors
Im“plant”ing of Mammalian Glycosyltransferase Gene into Plant Suspension-Cultured Cells Using Agrobacterium-Mediated Transformation
Transient Glyco-Engineering of N. benthamiana Aiming at the Synthesis of Multi-antennary Sialylated Proteins
Subcellular Targeting of Proteins Involved in Modification of Plant N- and O-Glycosylation
Assembly of Multigene Constructs Using Golden Gate Cloning
Strategies for Engineering Protein N-Glycosylation Pathways in Mammalian Cells