Stengel D.B., Connan S. (Eds.) Natural Products From Marine Algae. Methods and Protocols

Humana Press, 2015 — 439p. — (Methods in Molecular Biology 1308) — ISBN: 978-1-4939-2684-8 (eBook), 978-1-4939-2683-1 (Hardcover).This volume provides a fundamental overview of the current state of the art in natural products from marine algae, linking the complex and diverse natural resource with recent developments in extraction, analytical and bioactivity testing methodologies. Natural Products from Marine Algae: Methods and Protocols guides readers through protocols and techniques on algal biotechnology, metabolites, Solid-Liquid Extraction (SLE), Microwave Assisted Extraction (MAE), Liquid Chromatography, Gas Chromatography, Nuclear Magnetic Resonance Spectroscopy, Infra-red spectroscopy and Raman Spectroscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Natural Products from Marine Algae: Methods and Protocols hopes to aid scientists unravel and quantify algal chemical diversity and support further marine biotechnological developments.Marine Algae: a Source of Biomass for Biotechnological Applications
Structure and Function of Macroalgal Natural Products
Spectrophotometric Assays of Major Compounds Extracted from Algae
Extraction and Enrichment of Protein from Red and Green Macroalgae
Extraction and Purification of R-phycoerythrin from Marine Red Algae
Extraction and Analysis of Mycosporine-Like Amino Acids in Marine Algae
Extraction and Purification of Phlorotannins from Brown Algae
Enzyme-Enhanced Extraction of Antioxidant Ingredients from Algae
Microwave-Assisted Extraction of Fucoidan from Marine Algae
Extraction and Analysis of Oxylipins from Macroalgae Illustrated on the Example Gracilaria vermiculophylla
Lipids and Fatty Acids in Algae: Extraction, Fractionation into Lipid Classes, and Analysis by Gas Chromatography Coupled with Flame Ionization Detector (GC-FID)
HRMAS NMR Analysis of Algae and Identification of Molecules of Interest via Conventional 1D and 2D NMR: Sample Preparation and Optimization of Experimental Conditions
Extraction, Purification, and NMR Analysis of Terpenes from Brown Algae
Extraction, Isolation, and Identification of Sesquiterpenes from Laurencia Species
The Use of HPLC for the Characterization of Phytoplankton Pigments
Characterization of Phlorotannins from Brown Algae by LC-HRMS
Analysis of Betaines from Marine Algae Using LC-MS-MS
Analysis of Marine Biotoxins Using LC-MS/MS
Fucoidan Analysis by Tandem MALDI-TOF and ESI Mass Spectrometry
Determination of Substitution Patterns of Galactans from Green Seaweeds of the Bryopsidales