Kurien B.T., Scofield R.H. (Eds.) Detection of Blotted Proteins. Methods and Protocols

Humana Press, 2015 — 386p. — (Methods in Molecular Biology 1314) — ISBN: 978-1-4939-2718-0 (eBook), 978-1-4939-2717-3 (Hardcover).This detailed volume provides methods and techniques for detection after blotting. Chapters guide readers through a number of variations on the theme of protein transfer to solid support followed by detection, presenting adaptations of traditional techniques, and original methods of protein blotting. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and authoritative, Detection of Blotted Proteins: Methods and Protocols presents numerous techniques based on the Western blot, providing detailed, readily reproducible methods, tips, and alternatives directly and easily transferable to the laboratory setting.Western Blotting: Origin and Ascent of the Species
Methods to Concentrate Proteins for Protein Isolation, Proteomic, and Peptidomic Evaluation
Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose
Detection of Blotted Proteins: Not All Blockers Are Created Equal
Protein Stains to Detect Antigen on Membranes
Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting
Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation
Use of Nonradioactive Detection Method for North- and South-Western Blot
Immunoblotting Using Radiolabeled Reagents for Detection
Immunoblotting of Antigens: Whole, Strip, and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique
Detection of Protein Carbonyls by Means of Biotin Hydrazide–Streptavidin Affinity Methods
Direct Immunodetection of Antigens Within the Precast Polyacrylamide Gel
Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays
Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots
Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL Plex Immunoblotting in a Standard Proteomics Workflow
Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot Blots, and Immunohistochemistry
Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane
Sequential Use of Immunoblots for Characterization of Autoantibody Specificities
Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting
Application of Intermittent Microwave Irradiation to Western Blot Analysis