Lepenies B. (Ed.) Carbohydrate-Based Vaccines. Methods and Protocols

Humana Press, 2015 — 255p. — (Methods in Molecular Biology 1331) — ISBN: 978-1-4939-2874-3 (eBook), 978-1-4939-2873-6 (Hardcover).This volume summarizes current cutting-edge methods related to carbohydrate-based vaccines, from the identification of a suitable carbohydrate antigen via the preparation of glycoconjugate vaccines to the characterization of vaccine candidates for their use in pre-clinical and clinical studies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Carbohydrate-Based Vaccines: Methods and Protocols aims to be a valuable manual that will facilitate research in the field of carbohydrate-based vaccines.Carbohydrate-Based Vaccines: An Overview
Glycans as Vaccine Antigens and Adjuvants: Immunological Considerations
The Glycan Array Platform as a Tool to Identify Carbohydrate Antigens
Antibody-Carbohydrate Recognition from Docked Ensembles Using the AutoMap Procedure
Generation of Monoclonal Antibodies against Defined Oligosaccharide Antigens
Murine Whole-Blood Opsonophagocytosis Assay to Evaluate Protection by Antibodies Raised Against Encapsulated Extracellular Bacteria
Determination of N-linked Glycosylation in Viral Glycoproteins by Negative Ion Mass Spectrometry and Ion Mobility
N-Glycosylation Fingerprinting of Viral Glycoproteins by xCGE-LIF
Temporary Conversion of Protein Amino Groups to Azides: A Synthetic Strategy for Glycoconjugate Vaccines
Gold Nanoparticles as Carriers for Synthetic Glycoconjugate Vaccines
Identification and Characterization of Carbohydrate-Based Adjuvants
Characterization of Carbohydrate Vaccines by NMR Spectroscopy
Characterization of Capsular Polysaccharides and Their Glycoconjugates by Hydrodynamic Methods
Glycoconjugate Vaccines: The Regulatory Framework