Orzáez M., Sancho Medina M., Pérez-Payá E. (Eds.) Cyclin-Dependent Kinase (CDK) Inhibitors. Methods and Protocols

Humana Press, 2015 — 227p. — (Methods in Molecular Biology 1336) — ISBN: 978-1-4939-2926-9 (eBook), 978-1-4939-2925-2 (Hardcover).This volume contains a collection of relevant information for drug discovery in cell cycle research. Protocols to develop screening assays or to identify novel CDK inhibitors are discussed in the first part of the book. The second part of the book describes elaborate procedures to evaluate activity and mechanism of action of new and already identified CDK inhibitors. The third part of the book talks about protocols to evaluate metabolomics changes associated to inhibitor treatment. Drug delivery strategies focused on nanoparticle development to provide alternative internalization systems for increasing inhibitor efficacy are also described. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and thorough, Cyclin-Dependent Kinase (CDK) Inhibitors: Methods and Protocols is a useful tool for scientists interested in this research field.Immunoprecipitation of Cdk–Cyclin Complexes for Determination of Kinase Activity
Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation
Expression and Purification of Recombinant CDKs: CDK7, CDK8, and CDK9
Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination
Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors
Protein-Protein Interaction for the De Novo Design of Cyclin-Dependent Kinase Peptide Inhibitors
Identification of Cyclin A Binders with a Fluorescent Peptide Sensor
Cell Synchronization Techniques to Study the Action of CDK Inhibitors
Analysis of CDK Inhibitor Action on Mitochondria-Mediated Apoptosis
Evaluating the Effects of CDK Inhibitors in Ischemia–Reperfusion Injury Models
Assessing Cell Cycle Independent Function of the CDK Inhibitor p21^CDKN1A in DNA Repair
Drug Delivery Strategies of Chemical CDK Inhibitors
Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs
Evaluating Chemical CDK Inhibitors as Cell Death Inducers
Models for the Study of the Cross Talk Between Inflammation and Cell Cycle
Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors