Turksen K. (Ed.) Embryonic Stem Cell Protocols

Humana Press, 2016 — 3rd Ed. — 449p. — (Methods in Molecular Biology 1341) — ISBN: 978-1-4939-2954-2 (eBook), 978-1-4939-2953-5 (Hardcover).This extensive volume explores areas of intense activity related to the very early commitment of stem cells to particular lineages and the progression of differentiation to mature cell stages. Research on embryonic stem cells continues to move very quickly, thus the kinds of studies continue to expand and diversify, and methodologies are continuously being refined and improved, which this book reflects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and fully updated, Embryonic Stem Cell Protocols, Third Edition serves as an ideal reference for researchers investigating this rich area of study.Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition
A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells
Resolving Heterogeneity: Fluorescence-Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture
Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium
Generation of Embryonic Stem Cells in Rabbits
Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction
Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells
Expanding the Utility of FUCCI Reporters Using FACS-Based Omics Analysis
Selection of Antibodies Interfering with Cell Surface Receptor Signaling Using Embryonic Stem Cell Differentiation
Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells
Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells
Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells
Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation
Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers
Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells
From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells
In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor’s Production
Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes
Intracellular Calcium Measurements for Functional Characterization of Neuronal Phenotypes
In Vitro Differentiation of Pluripotent Stem Cells into Functional β Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets