Urban P.L., Chen Y.-C., Wang Y.-S. Time-Resolved Mass Spectrometry: From Concept to Applications

John Wiley & Sons, Ltd, 2016. – 388 p. – ISBN: 1118887328Time is an important factor in physical and natural sciences. It characterizes the progress of chemical and biochemical processes. Mass spectrometry provides the means to study molecular structures by detecting gas-phase ions with the unique mass-to-charge ratios. Time-resolved mass spectrometry (TRMS) allows one to differentiate between chemical states that can be observed sequentially at different time points. Real-time mass spectrometric monitoring enables recording data continuously with a specified temporal resolution. The TRMS approaches – introduced during the past few decades – have shown temporal resolutions ranging from hours down to microseconds and beyond.
This text covers the key aspects of TRMS. It introduces ion sources, mass analyzers, and interfaces utilized in time-resolved measurements; discusses the influence of data acquisition and treatment; finally, it reviews most prominent applications of TRMS – in the studies of reaction kinetics and mechanism, physicochemical phenomena, protein structure dynamics, biocatalysis, and metabolic profiling.
It will assist science and engineering students to gain a basic understanding of the TRMS concept, and to recognize its usefulness. In addition, it may benefit scientists who conduct molecular studies in the areas of chemistry, physics and biology.Ion Sources for Time-resolved Mass Spectrometry
Mass Analyzers for Time-resolved Mass Spectrometry
Interfaces for Time-resolved Mass Spectrometry
Balancing Acquisition Speed and Analytical Performance of Mass Spectrometry
Hyphenated Mass Spectrometric Techniques
Microfluidics for Time-resolved Mass Spectrometry
Quantitative Measurements by Mass Spectrometry
Data Treatment in Time-resolved Mass Spectrometry
Applications in Fundamental Studies of Physical Chemistry
Application of Time-resolved Mass Spectrometry in the Monitoring of Chemical Reactions
Applications of Time-resolved Mass Spectrometry in the Studies of Protein Structure Dynamics
Applications of Time-resolved Mass Spectrometry in Biochemical Analysis
Final Remarks