Owens R.J. (Ed.) Structural Proteomics. High-Throughput Methods

Humana Press, 2015 — 2nd Ed. — 375p. — (Methods in Molecular Biology 1261) — ISBN: 978-1-4939-2230-7 (eBook), 978-1-4939-2229-1 (Hardcover).This updated and expanded volume reflects the current state of the structural protein field with improved and refined protocols that have been applied to particularly challenging proteins, notably integral membrane proteins and multi-protein complexes. Structural Proteomics: High-Throughput Methods, Second Edition begins by exploring the resources available for curation, annotation, and structure prediction in silico, and continues with methods for sample preparation of both proteins and crystals, as well as structural characterization techniques. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and up-to-date, Structural Proteomics: High-Throughput Methods, Second Edition will aid researchers in expanding our knowledge of this vital and expansive area of protein science.Protein Structure Annotation Resources
PiMS: A Data Management System for Structural Proteomics
Prediction and Analysis of Intrinsically Disordered Proteins
Characterization and Production of Protein Complexes by Co-expression in Escherichia coli
The Production of Multiprotein Complexes in Insect Cells Using the Baculovirus Expression System
Production of Cell Surface and Secreted Glycoproteins in Mammalian Cells
Cell-Free Protein Synthesis Systems Derived from Cultured Mammalian Cells
Crystallization: Digging into the Past to Learn Lessons for the Future
Screening of Stable G-Protein-Coupled Receptor Variants in Saccharomyces cerevisiae
Cell-Free Expression of G-Protein-Coupled Receptors
GFP-Based Expression Screening of Membrane Proteins in Insect Cells Using the Baculovirus System
Methods for the Successful Crystallization of Membrane Proteins
Application of In Situ Diffraction in High-Throughput Structure Determination Platforms
CD Spectroscopy: An Essential Tool for Quality Control of Protein Folding
High-Throughput Studies of Protein Shapes and Interactions by Synchrotron Small-Angle X-Ray Scattering
Automated Structure Determination from NMR Spectra
Solid-State Nuclear Magnetic Resonance Spectroscopy for Membrane Protein Structure Determination
Native Mass Spectrometry: Towards High-Throughput Structural Proteomics