Gautier A., Hinner M.J. (Eds.) Site-Specific Protein Labeling. Methods and Protocols

Humana Press, 2015 — 267p. — (Methods in Molecular Biology 1266) — ISBN: 978-1-4939-2272-7 (eBook), 978-1-4939-2271-0 (Hardcover).This detailed volume provides in-depth protocols for protein labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Chapters provide protocols for labeling techniques and applications, with an additional focus on general background information on the design and generation of the organic molecules used for the labeling step. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Site-Specific Protein Labeling: Methods and Protocols provides a comprehensive overview on the most relevant and established labeling methodologies, and helps researchers to choose the most appropriate labeling method for their biological question.How FlAsH Got Its Sparkle: Historical Recollections of the Biarsenical-Tetracysteine Tag
Site-Specific Protein Labeling in the Pharmaceutical Industry: Experiences from Novartis Drug Discovery
Getting Across the Cell Membrane: An Overview for Small Molecules, Peptides, and Proteins
Considerations and Protocols for the Synthesis of Custom Protein Labeling Probes
2-Cyanobenzothiazole (CBT) Condensation for Site-Specific Labeling of Proteins at the Terminal Cysteine Residues
Fluorescent Labeling for Patch-Clamp Fluorometry (PCF) Measurements of Real-Time Protein Motion in Ion Channels
Fluorescent Labeling of SNAP-Tagged Proteins in Cells
HaloTag Technology for Specific and Covalent Labeling of Fusion Proteins
Ligation of Synthetic Peptides to Proteins Using Semisynthetic Protein trans-Splicing
Chemical-Tag Labeling of Proteins Using Fully Recombinant Split Inteins
Phage Selection Assisted by Sfp Phosphopantetheinyl Transferase-Catalyzed Site-Specific Protein Labeling
Site-Specific Biotinylation of Purified Proteins Using BirA
Site-Specific Labeling of Proteins via Sortase: Protocols for the Molecular Biologist
BONCAT: Metabolic Labeling, Click Chemistry, and Affinity Purification of Newly Synthesized Proteomes
Genetic Encoding of Unnatural Amino Acids for Labeling Proteins
Labeling Proteins by Affinity-Guided DMAP Chemistry
Ligand-Directed Tosyl Chemistry for Selective Native Protein Labeling In Vitro, In Cells, and In Vivo