Garcia-Pérez J.L. (Ed.) Transposons and Retrotransposons. Methods and Protocols

Humana Press, 2016 — 434p. — (Methods in Molecular Biology 1400) — ISBN: 978-1-4939-3372-3 (eBook), 978-1-4939-3370-9 (Hardcover).This volume covers the latest protocols designed to identify and characterize TEs in genomes, ancient or recently inserted. Additionally, this book includes a series of protocols designed to understand how host genomes act to regulate the activity of TEs, from elegant genetic mobilization assays to key biochemical methods. Finally, this book also includes chapters that describe how TEs can be used for biotechnological applications. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Transposons and Retrotransposons: Methods and Protocols aims to ensure successful results in the further study of this vital field.Study of Transposable Elements and Their Genomic Impact
Bacterial Group II Introns: Identification and Mobility Assay
In Silico Methods to Identify Exapted Transposable Element Families
Retrotransposon Capture Sequencing (RC-Seq): A Targeted, High-Throughput Approach to Resolve Somatic L1 Retrotransposition in Humans
Long Interspersed Element Sequencing (L1-Seq): A Method to Identify Somatic LINE-1 Insertions in the Human Genome
Combining Amplification Typing of L1 Active Subfamilies (ATLAS) with High-Throughput Sequencing
RNA-Seq Analysis to Measure the Expression of SINE Retroelements
Qualitative and Quantitative Assays of Transposition and Homologous Recombination of the Retrotransposon Tf1 in Schizosaccharomyces pombe
LINE Retrotransposition Assays in Saccharomyces cerevisiae
LINE-1 Cultured Cell Retrotransposition Assay
L1 Retrotransposition in Neural Progenitor Cells
Characterization of Engineered L1 Retrotransposition Events: The Recovery Method
SINE Retrotransposition: Evaluation of Alu Activity and Recovery of De Novo Inserts
The Engineered SVA Trans-mobilization Assay
Detection of LINE-1 RNAs by Northern Blot
Monitoring Long Interspersed Nuclear Element 1 Expression During Mouse Embryonic Stem Cell Differentiation
Immunodetection of Human LINE-1 Expression in Cultured Cells and Human Tissues
Cellular Localization of Engineered Human LINE-1 RNA and Proteins
Purification of L1-Ribonucleoprotein Particles (L1-RNPs) from Cultured Human Cells
Characterization of L1-Ribonucleoprotein Particles