Martin J. (Ed.) Poliovirus. Methods and Protocols

Humana Press, 2016 — 341p. — (Methods in Molecular Biology 1387) — ISBN: 978-1-4939-3292-4 (eBook), 978-1-4939-3291-7 (Hardcover).This volume describes the most common laboratory procedures for isolation, identification and characterization of polioviruses used in clinical and research laboratories. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Poliovirus: Methods and Protocols aims to ensure successful results in the further study of this vital field.An Introduction to Poliovirus: Pathogenesis, Vaccination, and the Endgame for Global Eradication
Poliovirus Laboratory Based Surveillance: An Overview
Isolation and Characterization of Enteroviruses from Clinical Samples
Isolation and Characterization of Poliovirus in Cell Culture Systems
Molecular Characterization of Polio from Environmental Samples: ISSP, The Israeli Sewage Surveillance Protocol
Quality Assurance in the Polio Laboratory. Cell Sensitivity and Cell Authentication Assays
A Transgenic Mouse Model of Poliomyelitis
Standardized Methods for Detection of Poliovirus Antibodies
Molecular Properties of Poliovirus Isolates: Nucleotide Sequence Analysis, Typing by PCR and Real-Time RT-PCR
Isolation and Characterization of Vaccine-Derived Polioviruses, Relevance for the Global Polio Eradication Initiative
Phylogenetic Analysis of Poliovirus Sequences
Generation of Infectious Poliovirus with Altered Genetic Information from Cloned cDNA
A Rapid Method for Engineering Recombinant Polioviruses or Other Enteroviruses
Methods to Monitor Molecular Consistency of Oral Polio Vaccine
Methods for the Quality Control of Inactivated Poliovirus Vaccines
Measuring Poliovirus Antigenicity by Surface Plasmon Resonance. Application for Potency Indicating Assays
Identification and Analysis of Antiviral Compounds Against Poliovirus