Feng Y., Zhang L. (Ed.) Long Non-Coding RNAs. Methods and Protocols

Humana Press, 2016 — 298p. — (Methods in Molecular Biology 1402) — ISBN: 978-1-4939-3378-5 (eBook), 978-1-4939-3376-1 (Hardcover).This volume assembles a broad spectrum of methods used in long non-coding RNAs (lncRNA) research, ranging from computational annotation of lncRNA genes to molecular and cellular analyses of the function of individual lncRNA. Long Non-Coding RNAs: Methods and Protocols also discusses methods used to study circular RNAs and RNA splicing, as well as influential findings on lncRNA in human diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Long Non-Coding RNAs: Methods and Protocols is a must-have for molecular biologists, cell and developmental biologists, specialists who conduct disease-oriented research, and bioinformatics experts who seek a better understanding on lncRNA expression and function by computational analysis of the massive sequencing data that are rapidly accumulating in recent years.LncRNA Pulldown Combined with Mass Spectrometry to Identify the Novel LncRNA-Associated Proteins
Cross-Linking Immunoprecipitation and qPCR (CLIP-qPCR) Analysis to Map Interactions Between Long Noncoding RNAs and RNA-Binding Proteins
Characterization of Long Noncoding RNA-Associated Proteins by RNA-Immunoprecipitation
Isolation of Protein Complexes Associated with Long Noncoding RNAs
Profiling Long Noncoding RNA Expression Using Custom-Designed Microarray
Long Noncoding RNA Expression Profiling Using Arraystar LncRNA Microarrays
Nuclear RNA Isolation and Sequencing
Targeted LncRNA Sequencing with the SeqCap RNA Enrichment System
ChIP-Seq: Library Preparation and Sequencing
Stellaris RNA Fluorescence In Situ Hybridization for the Simultaneous Detection of Immature and Mature Long Noncoding RNAs in Adherent Cells
Simultaneous RNA–DNA FISH
Visualizing Long Noncoding RNAs on Chromatin
Non-isotopic Method for In Situ LncRNA Visualization and Quantitation
Detection of Long Noncoding RNA Expression by Nonradioactive Northern Blots
Assessment of In Vivo siRNA Delivery in Cancer Mouse Models
Targeting Long Noncoding RNA with Antisense Oligonucleotide Technology as Cancer Therapeutics
Characterization of Circular RNAs
Methods for Characterization of Alternative RNA Splicing
NONCODEv4: Annotation of Noncoding RNAs with Emphasis on Long Noncoding RNAs
Computational Analysis of LncRNA from cDNA Sequences