Duque P. (Ed.) Environmental Responses in Plants. Methods and Protocols

Humana Press, 2016 — 387p. — (Methods in Molecular Biology 1398) — ISBN: 978-1-4939-3356-3 (eBook), 978-1-4939-3354-9 (Hardcover).This volume describes different up-to-date methodological approaches, ranging from physiological assays to imaging and molecular techniques, to study a wide variety of plant responses to environmental cues. Environmental Responses in Plants: Methods and Protocols is divided into four sections: Tropisms, Photoperiodism and Circadian Rhythms, Abiotic Stress Responses, and Plant-Pathogen Interactions. The chapters in these sections include detailed protocols to investigate some of the many key biological processes underlying plant environmental responses, mostly in the model organism Arabidopsis thaliana, but also in Physcomitrella patens and in different crop species such as rice, potato, barley, or tomato. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Environmental Responses in Plants: Methods and Protocols, is a great resource for plant physiologists, biochemists, and cell and molecular scientists interested in this exciting and fast-growing research topic.Hydrotropism: Analysis of the Root Response to a Moisture Gradient
Assessing Gravitropic Responses in Arabidopsis
Physiological Analysis of Phototropic Responses in Arabidopsis
Automatic Chloroplast Movement Analysis
Microscopic and Biochemical Visualization of Auxins in Plant Tissues
Immunolocalization of PIN and ABCB Transporters in Plants
Analysis of Circadian Leaf Movements
Sample Preparation of Arabidopsis thaliana Shoot Apices for Expression Studies of Photoperiod-Induced Genes
A Luciferase-Based Assay to Test Whether Gene Expression Responses to Environmental Inputs Are Temporally Restricted by the Circadian Clock
Identification of Arabidopsis Transcriptional Regulators by Yeast One-Hybrid Screens Using a Transcription Factor ORFeome
Monitoring Alternative Splicing Changes in Arabidopsis Circadian Clock Genes
Assessing the Impact of Photosynthetic Sugars on the Arabidopsis Circadian Clock
Assessing Protein Stability Under Different Light and Circadian Conditions
Screening for Abiotic Stress Tolerance in Rice: Salt, Cold, and Drought
Basic Techniques to Assess Seed Germination Responses to Abiotic Stress in Arabidopsis thaliana
Assessing Tolerance to Heavy-Metal Stress in Arabidopsis thaliana Seedlings
Assessing Drought Responses Using Thermal Infrared Imaging
Generating Targeted Gene Knockout Lines in Physcomitrella patens to Study Evolution of Stress-Responsive Mechanisms
Screening Stress Tolerance Traits in Arabidopsis Cell Cultures
Using Arabidopsis Protoplasts to Study Cellular Responses to Environmental Stress