Murhammer D.W. (Ed.) Baculovirus and Insect Cell Expression Protocols

Humana Press, 2016 — 3rd Ed. — 508p. — (Methods in Molecular Biology 1350) — ISBN: 978-1-4939-3043-2 (eBook), 978-1-4939-3042-5 (Hardcover).The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.Useful Tips, Widely Used Techniques, and Quantifying Cell Metabolic Behavior
Introduction to Baculovirus Molecular Biology
Baculovirus Transfer Vectors
Recombinant Baculovirus Isolation
Gene Expression in Mammalian Cells Using BacMam, a Modified Baculovirus System
Available Lepidopteran Insect Cell Lines
Lepidopteran Insect Cell Line Isolation from Insect Tissue
Development of Serum-Free Media for Lepidopteran Insect Cell Lines
Routine Maintenance and Storage of Lepidopteran Insect Cell Lines and Baculoviruses
Small-Scale Production of Recombinant Proteins Using the Baculovirus Expression Vector System
Recombinant Protein Production in Large-Scale Agitated Bioreactors Using the Baculovirus Expression Vector System
Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors
Protein Production with Recombinant Baculoviruses in Lepidopteran Larvae
Alternative Strategies for Expressing Multicomponent Protein Complexes in Insect Cells
Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression
Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression
Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression
Introduction to the Use of Baculoviruses as Biological Insecticides
Baculovirus Insecticide Production in Insect Larvae