Cao J. (Ed.) Breast Cancer. Methods and Protocols

Humana Press, 2016 — 332p. — (Methods in Molecular Biology 1406) — ISBN: 978-1-4939-3444-7 (eBook), 978-1-4939-3442-3 (Hardcover).This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer). Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and thorough, Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.Basic Histopathological Methods and Breast Lesion Types for Research
Clinical Applications for Immunohistochemistry of Breast Lesions
Immunohistochemistry for Triple-Negative Breast Cancer
In Situ Hybridization of Breast Cancer Markers
Evaluation of Human Epidermal Growth Factor Receptor 2 (HER2) Gene Status in Human Breast Cancer Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Specimens by Fluorescence In Situ Hybridization (FISH)
Quantification of mRNA Levels Using Real-Time Polymerase Chain Reaction (PCR)
Detection of miRNA in Cultured Cells or Xenograft Tissues of Breast Cancer
Pyrosequencing Analysis for Breast Cancer DNA Methylome
Vita-Assay Method of Enrichment and Identification of Circulating Cancer Cells/Circulating Tumor Cells (CTCs)
Breast Cancer Stem Cell Isolation
Cellular Apoptosis Assay of Breast Cancer
Assessment of Matrix Metalloproteinases by Gelatin Zymography
Assessment of Synthetic Matrix Metalloproteinase Inhibitors by Fluorogenic Substrate Assay
Determination of Breast Cancer Cell Migratory Ability
A Novel Collagen Dot Assay for Monitoring Cancer Cell Migration
Three-Dimensional Assay for Studying Breast Cancer Cell Invasion
A Combined Phagocytosis and Fluorescent Substrate Degradation Assay to Simultaneously Assess Cell Migration and Substrate Degradation
Analysis of Invadopodia Formation in Breast Cancer Cells
Patient-Derived Tumor Xenograft Models of Breast Cancer
Monitoring Phosphatidic Acid Signaling in Breast Cancer Cells Using Genetically Encoded Biosensors