McCoy C.E. (Ed.) Toll-Like Receptors. Practice and Methods

Humana Press, 2016 — 2nd Ed. — 455p. — (Methods in Molecular Biology 1390) — ISBN: 978-1-4939-3335-8 (eBook), 978-1-4939-3333-4 (Hardcover).This second edition volume expands on the first edition with new developments on Toll-Like Receptors (TLRs) controlling events such as cross-priming of associated pattern recognition receptors, post-transcriptional regulation, interaction with other cellular and biologic systems, and cancer progression. This book is divided into five sections: Part I outlines methods for TLR detection, interaction, and intracellular trafficking; Part II describes methods and assays to investigate how TLRs cross-prime other pattern recognition receptors, including intracellular DNA receptors and inflammasome formation; Part III highlights RNA regulation, detailing how TLRs can induce RNA transcripts and molecules such as lncRNAs and microRNAs; Part IV explores TLR detection and activation in systems such as epithelial barrier function, metabolism and the circadian clock, as well as cellular systems including T and B lymphocytes; and Part V describes models to delineate the role of TLRs in diseases such as dermatitis, arthritis, and gastric cancer. Written in the highly successful Methods in Molecular Biology series format, each chapter contains a summary, a list of required materials, step-by-step, readily reproducible laboratory protocols, useful notes to investigate TLRs in cell culture, systems and disease, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Toll-Like Receptors: Methods and Protocols, Second Edition is a valuable resource to any immunologist, molecular or medical biologist working in a laboratory setting. It will add skill to both students and the more advanced molecular biologist who wishes to learn a new technique or move to a different area within their current repertoire of practical knowledge.Toll-Like Receptors: Ligands, Cell-Based Models, and Readouts for Receptor Action
Bioinformatic Analysis of Toll-Like Receptor Sequences and Structures
Toll-Like Receptor Interactions Measured by Microscopic and Flow Cytometric FRET
Using Confocal Microscopy to Investigate Intracellular Trafficking of Toll-Like Receptors
Assessing the Inhibitory Activity of Oligonucleotides on TLR7 Sensing
Methods for Delivering DNA to Intracellular Receptors
Detection of Interaction Between Toll-Like Receptors and Other Transmembrane Proteins by Co-immunoprecipitation Assay
Flow Cytometry-Based Bead-Binding Assay for Measuring Receptor Ligand Specificity
Measuring Monomer-to-Filament Transition of MAVS as an In Vitro Activity Assay for RIG-I-Like Receptors
Co-transcriptomic Analysis by RNA Sequencing to Simultaneously Measure Regulated Gene Expression in Host and Bacterial Pathogen
Simple Methods to Investigate MicroRNA Induction in Response to Toll-Like Receptors
Determining the Function of Long Noncoding RNA in Innate Immunity
Analysis of Post-transcriptional Gene Regulation of Nod-Like Receptors via the 3′UTR
TLR Function in Murine CD4⁺ T Lymphocytes and Their Role in Inflammation
Analysis by Flow Cytometry of B-Cell Activation and Antibody Responses Induced by Toll-Like Receptors
Toll-Like Receptor-Dependent Immune Complex Activation of B Cells and Dendritic Cells
Analysis of TLR-Induced Metabolic Changes in Dendritic Cells Using the Seahorse XF^e96 Extracellular Flux Analyzer
Toll-Like Receptor Signalling and the Control of Intestinal Barrier Function
Understanding the Role of Cellular Molecular Clocks in Controlling the Innate Immune Response
Methods to Investigate the Role of Toll-Like Receptors in Allergic Contact Dermatitis