Luthra R., Singh R.R., Patel K.P. (Eds.) Clinical Applications of PCR

Humana Press, 2016 — 3rd Ed. — 191p. — (Methods in Molecular Biology 1392) — ISBN: 978-1-4939-3360-0 (eBook), 978-1-4939-3358-7 (Hardcover).This updated volume explores a wide variety of clinical applications of PCR such as detecting DNA methylation, detection of viruses and protozoa in infectious diseases, estimation of gene copy number aberrations, primer extension coupled with mass spectroscopy, and high throughput NGS techniques. The application of PCR has shown incredible value in the study of genomics and transcriptomics, not only for discovery but also for routine clinical applications, and it forms the cornerstone of personalized medicine. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and authoritative, Clinical Applications of PCR, Third Edition serves as an ideal guide for researchers aiming to understand the principles behind each application and for their implementation in the laboratory.A Targeted Q-PCR-Based Method for Point Mutation Testing by Analyzing Circulating DNA for Cancer Management Care
COLD-PCR: Applications and Advantages
PCR-Based Detection of DNA Copy Number Variation
Emulsion PCR: Techniques and Applications
Digital PCR: Principles and Applications
Quantitative PCR for Plasma Epstein-Barr Virus Loads in Cancer Diagnostics
High-Resolution Melt Curve Analysis in Cancer Mutation Screen
Locked Nucleic Acid Probes (LNA) for Enhanced Detection of Low-Level, Clinically Significant Mutations
Genotyping of Frequent Mutations in Solid Tumors by PCR-Based Single-Base Extension and MassARRAY Analysis
Microfluidics-Based PCR for Fusion Transcript Detection
Polymerase Chain Reaction Diagnosis of Leishmaniasis: A Species-Specific Approach
Detection of Trypanosoma cruzi by Polymerase Chain Reaction
PCR Techniques in Next-Generation Sequencing
Single-Cell Quantitative PCR: Advances and Potential in Cancer Diagnostics
Quantitative Real-Time PCR: Recent Advances
PCR Techniques in Characterizing DNA Methylation