Fett-Neto A.G. (Ed.) Biotechnology of Plant Secondary Metabolism. Methods and Protocols

Humana Press, 2016 — 180p. — (Methods in Molecular Biology 1405) — ISBN: 978-1-4939-3393-8 (eBook), 978-1-4939-3391-4 (Hardcover).This volume describes up-to-date techniques for improved production of secondary metabolites of economic interest using field and laboratory methods. Biotechnology of Plant Secondary Metabolism: Methods and Protocols explores different secondary metabolite classes, whole-plant and cell/organ culture systems, and environmental and genetic transformation-based modulation of biochemical pathways. Special focus is given to cell and tissue specific metabolism, metabolite transport, microRNA-based technology, heterologous systems expression of enzymes and pathways leading to products of interest, as well as applications using both model and non-model plant species. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Practical and cutting-edge, Biotechnology of Plant Secondary Metabolism: Methods and Protocols is a great resource for scientists of interdisciplinary fields--plant science, plant physiology, pharmacy, molecular biology, biochemistry, bioengineering, and forestry--in reaching their goals of producing plant biochemicals in a sustainable and efficient manner, while minimizing impacts to the environment and providing the required quantities of these commodities to industry.Elicitation Approaches for Withanolide Production in Hairy Root Culture of Withania somnifera (L.) Dunal
Stimulant Paste Preparation and Bark Streak Tapping Technique for Pine Oleoresin Extraction
A Modified Protocol for High-Quality RNA Extraction from Oleoresin-Producing Adult Pines
Collection of Apoplastic Fluids from Arabidopsis thaliana Leaves
From Plant Extract to a cDNA Encoding a Glucosyltransferase Candidate: Proteomics and Transcriptomics as Tools to Help Elucidate Saponin Biosynthesis in Centella asiatica
Production of Recombinant Caffeine Synthase from Guarana (Paullinia cupana var. sorbilis) in Escherichia coli
Heterologous Expression and Characterization of Mimosinase from Leucaena leucocephala
Production of Aromatic Plant Terpenoids in Recombinant Baker’s Yeast
Purification of a Recombinant Polyhistidine-Tagged Glucosyltransferase Using Immobilized Metal-Affinity Chromatography (IMAC)
A Western Blot Protocol for Detection of Proteins Heterologously Expressed in Xenopus laevis Oocytes
Laser Capture Microdissection: Avoiding Bias in Analysis by Selecting Just What Matters
Analytical and Fluorimetric Methods for the Characterization of the Transmembrane Transport of Specialized Metabolites in Plants
Protoplast Transformation as a Plant-Transferable Transient Expression System
Design, Construction, and Validation of Artificial MicroRNA Vectors Using Agrobacterium-Mediated Transient Expression System
Knockdown of Polyphenol Oxidase Gene Expression in Potato (Solanum tuberosum L.) with Artificial MicroRNAs