Hewitson T.D., Smith E.R., Holt S.G. (Eds.) Kidney Research. Experimental Protocols

Humana Press, 2016 — 2nd Ed. — 292p. — (Methods in Molecular Biology 1397) — ISBN: 978-1-4939-3353-2 (eBook), 978-1-4939-3351-8 (Hardcover).This second edition expands upon the previous volume with additional emphasis on recent innovation in basic renal research and has a more holistic approach on associated disorders such as complications associated with peritoneal dialysis, ischemic acute kidney injury, the sympathetic nervous system, and vascular calcification. The book is divided into five parts: Part I provides a number of in vitro, in vivo, and ex vivo models of kidney disease and associated complications; Part II looks at recent advances in imaging techniques; Part III covers recent developments in studying metabolism in renal ischemia and reperfusion; Part IV addresses study and measurement of vascular calcification; and Part V explores analytical techniques that are both topical and of widespread relevance to the study of experimental renal disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Kidney Research: Experimental Protocols, Second Edition is a valuable collection of protocols useful to new and experienced researchers who are interested in the field of Nephrology.Isolation and Primary Culture of Murine Podocytes with Proven Origin
Propagation and Culture of Human Renal Fibroblasts
Isolation and Propagation of Rat Peritoneal Mesothelial Cells
Rat Models of Acute and/or Chronic Peritoneal Injuries Including Peritoneal Fibrosis and Peritoneal Dialysis Complications
Renal Sympathetic Denervation in Rats
Decellularization of Rat Kidneys to Produce Extracellular Matrix Scaffolds
Use of Cationized Ferritin Nanoparticles to Measure Renal Glomerular Microstructure with MRI
Biopsychronology: A Method Using Live Tissue Staining to Image Cell Function in the Kidney
Prolonged and Continuous Measurement of Kidney Oxygenation in Conscious Rats
Magnetic Resonance Imaging (MRI) Analysis of Ischemia/Reperfusion in Experimental Acute Renal Injury
Assessment of Renal Hemodynamics and Oxygenation by Simultaneous Magnetic Resonance Imaging (MRI) and Quantitative Invasive Physiological Measurements
Intravital Multiphoton Imaging of the Kidney: Tubular Structure and Metabolism
Vascular Calcification in Uremia: New-Age Concepts about an Old-Age Problem
An In Vitro Murine Model of Vascular Smooth Muscle Cell Mineralization
The Isolation and Quantitation of Fetuin-A-Containing Calciprotein Particles from Biological Fluids
Combining Near Infrared Fluorescent Imaging for Calcification and Inflammation in Vascular Tissue Samples Ex Vivo
Laser Capture Microdissection of Archival Kidney Tissue for qRT-PCR
Using Phos-Tag in Western Blotting Analysis to Evaluate Protein Phosphorylation
Qualitative and Quantitative Analysis of Histone Deacetylases in Kidney Tissue Sections