Broshanan A.J. (Ed.) Superantigens. Methods and Protocols

Humana Press, 2016 — 228p. — (Methods in Molecular Biology 1396) — ISBN: 978-1-4939-3344-0 (eBook), 978-1-4939-3342-6 (Hardcover).This volume combines protocols that encompass the true variety of investigation done on superantigens in the fields of microbiology, immunology, molecular biology, biochemistry, and cellular biology, with a strong focus on disease models utilized to determine the role of superantigens in human disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Thorough and cutting-edge, Superantigens: Methods and Protocols contains a detailed and wide breadth of subject coverage that any scientist, clinician, or industry professional interested in this field will find valuable.Animal Models Used to Study Superantigen-Mediated Diseases
Identification, Purification, and Characterization of Staphylococcal Superantigens
Strain Discrimination of Staphylococcus aureus Using Superantigen Profiles
Crystallization and Structure Determination of Superantigens and Immune Receptor Complexes
Lipopolysaccharide-Induced Toxic Shock Syndrome in Rabbits
Aortic Valve Damage for the Study of Left-Sided, Native Valve Infective Endocarditis in Rabbits
Rabbit Model for Superantigen-Mediated Lethal Pulmonary Disease
Nasopharyngeal Infection of Mice with Streptococcus pyogenes and In Vivo Detection of Superantigen Activity
Mini-Osmotic Pump Infusion Model to Investigate the Systemic Effects of Chronic Continuous Exposure to Staphylococcal Superantigen in Mice
A Sublethal Swine Model for Defining In Vivo Superantigen-Induced Responses Following Exposure to Staphylococcal Enterotoxin B
Monkey Feeding Assay for Testing Emetic Activity of Staphylococcal Enterotoxin
Use of an Ex Vivo Porcine Mucosal Model to Study Superantigen Penetration
Protocol for Examining Human Vaginal Epithelial Cell Signaling in Response to Staphylococcal Superantigens
Organ Culture as a Model System for Studies on Enterotoxin Interactions with the Intestinal Epithelium
Quantification of a Selective Expansion of T Cell Receptor Vβ by Superantigen Using Real-Time PCR
Induction of Human Regulatory T Cells with Bacterial Superantigens
Construction of Recombinant Single Chain Variable Fragment (ScFv) Antibody Against Superantigen for Immunodetection Using Antibody Phage Display Technology