Turksen K., Nagy A. (Eds.) Induced Pluripotent Stem (iPS) Cells. Methods and Protocols

Humana Press, 2016 — 496p. — (Methods in Molecular Biology 1357) — ISBN: 978-1-4939-3055-5 (eBook), 978-1-4939-3054-8 (Hardcover).This volume aims to be a collection of essential protocols in iPS cell field. Induced Pluripotent Stem (iPS) Cells: Methods and Protocolsguides readers through multiple facets of stem cell biology, lineage commitment and differentiation. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Induced Pluripotent Stem (iPS) Cells: Methods and Protocols provides protocols that can be used by both experts and novices interested in stem cells.The piggyBac Transposon as a Platform Technology for Somatic Cell Reprogramming Studies in Mouse
Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells Using Sendai Virus
Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton’s Jelly into Induced Pluripotent Stem Cells
Very Rapid and Efficient Generation of Induced Pluripotent Stem Cells from Mouse Pre-B Cells
Generation of iPS Cells from Human Peripheral Blood Mononuclear Cells Using Episomal Vectors
Enhancing Induced Pluripotent Stem Cell Generation by MicroRNA
Generation of Partially Reprogrammed Cells and Fully Reprogrammed iPS Cells by Plasmid Transfection
Using Oct4:MerCreMer Lineage Tracing to Monitor Endogenous Oct4 Expression During the Reprogramming of Fibroblasts into Induced Pluripotent Stem Cells (iPSCs)
Inducible Transgene Expression in Human iPS Cells Using Versatile All-in-One piggyBac Transposons
Generation and Characterization of Rat iPSCs
Generation of Induced Pluripotent Stem Cells in Rabbits
Cynomolgus Monkey Induced Pluripotent Stem Cells Generated By Using Allogeneic Genes
Induced Pluripotent Stem Cells from Nonhuman Primates
Computational Biology Methods for Characterization of Pluripotent Cells
cGMP-Compliant Expansion of Human iPSC Cultures as Adherent Monolayers
Analysis of the Mitochondrial DNA and Its Replicative Capacity in Induced Pluripotent Stem Cells
Selection of Phage Display Peptides Targeting Human Pluripotent Stem Cell-Derived Progenitor Cell Lines
piggyBac Transposon Mediated Reprogramming and Flow Cytometry Analysis of CD44 and ICAM1 Cell-Surface Marker Changes
Generation and In Vitro Expansion of Hepatic Progenitor Cells from Human iPS Cells
Induced Pluripotent Stem (iPS) Cell Culture Methods and Induction of Differentiation into Endothelial Cells