Cuturi M.C., Anegon I. (Eds.) Suppression and Regulation of Immune Responses. Methods and Protocols

Humana Press, 2011 — 473p. — (Methods in Molecular Biology 677) — ISBN: 978-1-60761-869-0 (eBook), 978-1-60761-868-3 (Hardcover).Over the past several years, a high diversity of regulatory cells and suppressive molecules has taken centre stage in the field of immunoregulation.In Suppression and Regulation of Immune Responses: Methods and Protocols, expert researchers highlight recent advances in the identification, characterization, and generation of regulatory cells not only of the T cell lineage but also of other origins such as B, NK, myeloid, and dendritic cells, as well as the role of several suppressive molecules in immunoregulation.Particular emphasis is placed on the characterization of the molecular mechanisms and the therapeutic applications of regulatory cells and molecules in human diseases.Written as a volume in the highly successful Methods in Molecular Biology series, this work provides the kind of detailed description and implementation advice that is crucial for getting optimal results.
Comprehensive and cutting-edge, Suppression and Regulation of Immune Responses: Methods and Protocols serves as a key reference for scientists seeking a way toward greater control over the enormous power of the complex and vital immune system.Natural and Induced T CD4+CD25+FOXP3+ Regulatory T Cells
Polyclonal Expansion of Human CD4⁺CD25⁺ Regulatory T Cells
Methods for In Vitro Generation of Human Type 1 Regulatory T Cells
Ex Vivo Generation of Regulatory T Cells: Characterization and Therapeutic Evaluation in a Model of Chronic Colitis
Phenotypic and Functional Characterization of CD8⁺ T Regulatory Cells
Regulatory CD4^– CD8^– Double Negative T Cells
Identifying Regulatory B Cells (B10 Cells) that Produce IL-10 in Mice
DCs in Immune Tolerance in Steady-State Conditions
Plasmacytoid Dendritic Cells in Tolerance
In Vitro-Generated DC with Tolerogenic Functions: Perspectives for In Vivo Cellular Therapy
Preparation of Mouse Bone Marrow-Derived Dendritic Cells with Immunoregulatory Properties
Myeloid-Derived Suppressor Cells: Characterization and Expansion in Models of Endotoxemia and Transplantation
Human Regulatory Macrophages
NKT and Tolerance
Antiinflammatory and Immunosuppressive Functions of Mast Cells
A Mesenchymal Stem Cell Potency Assay
In Vitro Analyses of the Immunosuppressive Properties of Neural Stem/Progenitor Cells Using Anti-CD3/CD28-Activated T Cells
Immunoregulatory Properties of Heme Oxygenase-1
Indoleamine 2,3-Dioxygenase and Regulatory Function: Tryptophan Starvation and Beyond
Regulatory T Cell Enrichment by IFN-γ Conditioning