Bouchonnet S. Introduction to GC-MS Coupling

Taylor & Francis Group, LLC, 2013. – 228 p. – ISBN: 1466572515Although GC-MS (gas chromatography–mass spectrometry) finds applications in fields as diverse as the food processing industry, medicine, pharmacology, and environmental analysis, the few works that are dedicated to this use of mass spectrometry are generally highly complex and theoretical.
Emphasizing the practical aspects of GC-MS, without neglecting the fundamental theory, Introduction to GC-MS Coupling addresses both novice and experienced users of this technique. It presents GC-MS in a clear, instructive way and proposes solutions for the difficulties classically encountered by users.
The book begins with the core principles of gas chromatography and its specific uses with MS detectors. It discusses generalities of mass spectrometry, including the various types of MS detectors and insight into the vacuum necessary for efficient operation. Chapters cover the types of analyzers used in GC-MS and their functioning principles, with a focus on the commonly used quadrupolar analyzers, as well as the implementation, advantages, and limits of various modes of acquisition in GC-MS. The text also compares performance and limitations of quadrupolar analyzers.
The author includes a full chapter on quantification using GC-MS, a topic that can be puzzling for many chemists. Encouraging a critical approach to databases, he compares laboratory-made and commercial mass spectra databases, and describes a database research algorithm. The final chapter examines mass spectra interpretation, covering chemistry concepts such as inductive and mesomeric effects required to understand dissociation pathways, and presents a global strategy for mass spectra interpretation.
Contents
Concepts of Gas Chromatography
General Points
Instrumentation
Fast Chromatography
Multidimensional Chromatography
Introduction to Mass Spectrometry: General Points
Mass Spectrometer Components
Vacuum
Detectors
Ion Production in GC-MS: Sources
Definitions and General Points
Electron Ionization
Chemical Ionization
Ion Separation: Analyzers
General Points
Technical Features
Quadrupoles
Ion Traps
Time-of-Flight Analyzers
Magnetic Analyzers
Acquisition Modes in GC-MS
Definitions
Full Scan Analysis
SIR, SIM, and SIS Modes
Tandem Mass Spectrometry (MS/MS)
Comparison of Quadrupolar Analyzer Performances: Criteria for Choosing an Analyzer
Comparison Criteria
Spectral Repeatability and Autoprotonation
Response Functions and Limits of Detection
Chromatographic Profiles
Maintenance and Robustness
Choosing a Quadrupolar Analyzer
Quantification by GC-MS
External Standard Quantification
Internal Standard Quantification
Method Validation
Spectra Databases for GC-MS
Limitations of Mass Spectra Interpretation
Spectra Databases
Research Algorithm
Results Display
Research Parameters
Databases and Mass Spectra Interpretation
Other Uses of Databases
Introduction to Mass Spectra Interpretation
Chemistry Principles Fundamental to Mass Spectra Interpretation.
Dissociation Mechanisms and Interpretation of Mass Spectra in Electron Ionization
Dissociation Mechanisms in Positive Chemical Ionization
Dissociation Mechanisms in Negative Chemical Ionization
Mass Spectra Interpretation Strategy