May M.J. (Ed.) NF-kappa B. Methods and Protocols

Humana Press, 2015. — 668 p. — (Methods in Molecular Biology 1280). — ISBN: 978-1-4939-2422-6 (eBook), 978-1-4939-2421-9 (Hardcover).Focusing on essential insight into the methods and techniques required to dissect the complex mechanisms of NF-κB activation, regulation, and function.NF-kappaB: Methods and Protocolsguides readers through standard approaches to detect NF-κB pathway activation, detection and analysis ofNF-κB signaling, and methods to study the control ofNF-κBsignaling. As a volume in the highly successful Methods in Molecular Biologyseries, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and authoritative, NF-KappaB: Methods and Protocolsprovides a timely and invaluable resource for researchers seeking to perform experiments aimed at understanding the role of NF-κB signaling in health and disease.Electrophoretic Mobility Shift Assay Analysis of NF-κB DNA Binding.
Detection of IκB Degradation Dynamics and IκB-α Ubiquitination.
Measurement of NF-κB Transcriptional Activity and Identification of NF-κB.
Assessing Sites of NF-κB DNA Binding Using Chromatin Immunoprecipitation.
IKK Kinase Assay for Assessment of Canonical NF-κB Activation in Neurons.
Analysis of Epidermal Growth Factor-Induced NF-κB Signaling.
Methods to Assess the Activation of the Alternative (Noncanonical) NF-κB Pathway by Non-death TNF Receptors.
Systematic Detection of Noncanonical NF-κB Activation.
Noncanonical NF-κB Activation and SDF-1 Expression in Human Endothelial Cells.
Stable Reconstitution of IKK-Deficient Mouse Embryonic Fibroblasts.
Dissecting NF-κB Signaling Induced by Genotoxic Agents via Genetic Complementation of NEMO-Deficient 1.3E2 Cells.
Visualizing TCR-Induced POLKADOTS Formation and NF-κB Activation in the D10 T-Cell Clone and Mouse Primary Effector T Cells.
Detection of Recombinant and Cellular MALT1 Paracaspase Activity.
TRAF Protein Function in Noncanonical NF-κB Signaling.
Roles of c-IAP Proteins in TNF Receptor Family Activation of NF-κB Signaling.
Elucidating Dynamic Protein–Protein Interactions and Ubiquitination in NF-κB Signaling Pathways.
Immunoblot Analysis of Linear Polyubiquitination of NEMO.
In Vitro Detection of NEMO–Ubiquitin Binding Using DELFIA and Microscale Thermophoresis Assays.
Use of Fluorescence Spectroscopy for Quantitative Investigations of Ubiquitin Interactions with the Ubiquitin-Binding Domains of NEMO.
Generation of a Proteolytic Signal: E3/E2-Mediated Polyubiquitination of IκBα.
Control of NF-κB Subunits by Ubiquitination.
Methodology to Study NF-κB/RelA Ubiquitination In Vivo.
Using Sequential Immunoprecipitation and Mass Spectrometry to Identify Methylation of NF-κB.
Methods to Detect NF-κB Acetylation and Methylation.
A Method for the Quantitative Analysis of Stimulation-Induced Nuclear Translocation of the p65 Subunit of NF-κB from Patient-Derived Dermal Fibroblasts.
Methods for Assessing the In Vitro Transforming Activity of NF-κB Transcription Factor c-Rel and Related Proteins.
Using RNA Interference in Lung Cancer Cells to Target the IKK-NF-κB Pathway.
Immunohistochemical Analysis of NF-κB in Human Tumor Tissue.
Assessment of Canonical NF-κB Activity in Canine Diffuse Large B-Cell Lymphoma.
NEMO-Binding Domain Peptide Inhibition of Inflammatory Signal-Induced NF-κB Activation In Vivo.
Regulation of NF-κB Signaling in Osteoclasts and Myeloid Progenitors.
NF-κB Activation with Aging: Characterization and Therapeutic Inhibition.
The “Sneaking-Ligand” Approach: Cell-Type Specific Inhibition of the Classical NF-κB Pathway.
Sneaking-Ligand Fusion Proteins Attenuate Serum Transfer Arthritis by Endothelium-Targeted NF-κB Inhibition.
Analysis of NF-κB Activation in Mouse Intestinal Epithelial Cells.
Characterizing the DNA Binding Site Specificity of NF-κB with Protein-Binding Microarrays (PBMs).
Methods for Analyzing the Evolutionary Relationship of NF-κB Proteins Using Free, Web-Driven Bioinformatics and Phylogenetic Tools.
Studying NF-κB Signaling with Mathematical Models.