Valla S., Lale R. (Eds.) DNA Cloning and Assembly Methods

Humana Press, 2014 — 303p. — (Methods in Molecular Biology 1116) — ISBN: 978-1-62703-764-8 (eBook), 978-1-62703-763-1 (Hardcover).In DNA Cloning and Assembly Methods, expert researchers in the field detail many of the methods which are now commonly used for DNA cloning and make cloning procedures faster, more reliable and also suitable for high-throughput handling. These include methods and protocols that are based on several mechanisms including type II and IIS restriction enzymes, single stranded annealing, sequence overlap, and recombination.
With additional chapters on software programs that are suitable for primer design, a feature crucial for the functionality of the described methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, DNA Cloning and Assembly Methods seeks to provide scientist with a valuable and useful resource for wet lab researchers within life sciences.BioBrick Assembly Standards and Techniques and Associated Software Tools
Plasmid Construction by SLIC or Sequence and Ligation-Independent Cloning
Quick and Clean Cloning
Hierarchical Ligation-Independent Assembly of PCR Fragments
USER-Derived Cloning Methods and Their Primer Design
Application of the Restriction-Free (RF) Cloning for Multicomponents Assembly
A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform
Circular Polymerase Extension Cloning
Golden Gate Cloning
Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
FX Cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
Minimum GC-Rich Sequences for Overlap Extension PCR and Primer Annealing
Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
Application of In-Fusion Cloning for the Parallel Construction of E. coli Expression Vectors
Seamless Ligation Cloning Extract (SLiCE) Cloning Method
j5 DNA Assembly Design Automation
FastPCR Software for PCR, In Silico PCR, and Oligonucleotide Assembly and Analysis