Kuo J. (Ed.) Electron Microscopy. Methods and Protocols

Humana Press, 2014 — 3rd Ed. — 799p. — (Methods in Molecular Biology 1117) — ISBN: 978-1-62703-776-1 (eBook), 978-1-62703-775-4 (Hardcover).This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.Conventional Specimen Preparation Techniques for Transmission Electron Microscopy of Cultured Cells
Microwave-Assisted Processing and Embedding for Transmission Electron Microscopy
Processing Plant Tissues for Ultrastructural Study
Staining Sectioned Biological Specimens for Transmission Electron Microscopy: Conventional and En Bloc Stains
Freeze Fracture and Freeze Etching
Conventional Specimen Preparation Techniques for Scanning Electron Microscopy of Biological Specimens
High-Pressure Freezing: Current State and Future Prospects
Cryo-fixation by Self-Pressurized Rapid Freezing
Cryo-electron Microscopy of Vitreous Sections
Negative Staining and Cryo-negative Staining: Applications in Biology and Medicine
Electron Microscopy of Microtubule Cytoskeleton Assembly In Vitro
Cryosectioning Fixed and Cryoprotected Biological Material for Immunocytochemistry
Analysis of Specificity in Immunoelectron Microscopy
Cryo-electron Microscopy of Membrane Proteins
Tracking DNA and RNA Sequences at High Resolution
Visualization of DNA and Protein–DNA Complexes with Atomic Force Microscopy
Biological Applications of Phase-Contrast Electron Microscopy
Single Particle Cryo-electron Microscopy and 3-D Reconstruction of Viruses
Electron Tomography for Organelles, Cells, and Tissues