Chillón M., Bosch A. (Eds.) Adenovirus: Methods and Protocols

Humana Press, 2014. — 247 p. — ISBN: 1627036784
Series: Methods in Molecular Biology, №1089Reflecting the development of powerful new tools and high-throughput methods to analyze adenoviral particles and their interactions with host cells, the third edition of Adenovirus Methods and Protocols calls upon experts in the field to convey advances in molecular biology, genomics and proteomics, imaging, and bioinformatics. Beginning with cryo-electron microscopy, atomic force microscopy, and mass spectrometry for a high resolution image and characterization of the virion, this detailed book then continues with capsid modifications and viral-like particles as promising alternatives to classical adenovirus vectors, and the study of adenovirus in host interactions in vitro at the cellular level as well as in vivo in animal models. Finally, the volume concludes with an extensive update of the most efficient protocols to generate, amplify, and/or purify, at small and large scale, standard human Ad5 as well as non-human, chimeric, and helper-dependent adenovirus vectors. Written in the greatly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Comprehensive and cutting-edge, Adenovirus Methods and Protocols, Third Edition serves as an ideal guide for scientists continuing to research this highly valuable viral tool.Biophysical Methods to Monitor Structural Aspects of the Adenovirus Infectious Cycle
Proteome Analysis of Adenovirus Using Mass Spectrometry
Capsid Modification Strategies for Detargeting Adenoviral Vectors
Use of Dodecahedron VLPs as an Alternative to the Whole Adenovirus
Study of Adenovirus and CAR Axonal Transport in Primary Neurons
The Use of Chromatin Immunoprecipitation (ChIP) to Study the Binding of Viral Proteins to the Adenovirus Genome In Vivo
DNA Microarray to Analyze Adenovirus–Host Interactions
Determination of the Transforming Activities of Adenovirus Oncogenes
Oncolytic Adenovirus Characterization: Activity and Immune Responses
The Analysis of Innate Immune Response to Adenovirus Using Antibody Arrays
Engineering Adenovirus Genome by Bacterial Artificial Chromosome (BAC) Technology
Construction, Production, and Purification of Recombinant Adenovirus Vectors
Scalable Production of Adenovirus Vectors
Canine Adenovirus Downstream Processing Protocol
Production of High-Capacity Adenovirus Vectors
Production of Chimeric Adenovirus