Mondal P.P., Diaspro A. Fundamentals of Fluorescence Microscopy: Exploring Life with Light

Springer, 2014. - 218 pp.
This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors.
The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stokes shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices.Basics and Fundamentals
Ray Optics, Wave Optics and Imaging System Design.
Basics of Electromagnetic Theory for Fluorescence Microscopy.
Electric Field Effects in Optical Microscopy Systems.
Quantum Description of Radiation Field and Optical Microscopy.
Molecular Physics of Fluorescent Markers.
Advanced Imaging
Basics of Fluorescence and Photophysics.
General Fluorescence Imaging Techniques.
Multiphoton Fluorescence Microscopy.
Super-resolution Fluorescence Microscopy.
Image Reconstruction for Fluorescence Microscopy.
Future Prespective of Fluorescence Microscopy.