Kneale G.G. (ed.) DNA-Protein Interactions. Principles and Protocols

Humana Press, 1994. - 425 p. ISBN: 0896032566
Series: Methods in Molecular Biology, №30DNase I Footprinting
Footprinting with Exonuclease III
Hydroxyl Radical Footprinting
Hydroxyl Radical Interference
1,10-Phenanthroline-Copper Ion Nuclease Footprinting of DNA-Protein Complexes in Situ Following Mobility-Shift Electrophoresis Assays
Identification of Protein-DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference
Diethyl Pyrocarbonate as a Probe of Protein-DNA Interactions
Osmium Tetroxide Modification and the Study of DNA-Protein Interactions
Diffusible Singlet Oxygen as a Probe of DNA Deformation
Ethylation Interference
Uranyl Photofootprinting of DNA-Protein Complexes
Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes
Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes
Cloning and Expression of DNA Binding Domains Using PCR
Overexpression and Purification of Eukaryotic Transcription Factors as Glutathione-S-Transferase Fusions in E. coli
Site-Directed Mutagenesis by the Cassette Method
Site-Directed and Site-Saturation Mutagenesis Using Oligonucleotide Primers
UV Laser-Induced Protein-DNA Crosslinking
Ultraviolet Crosslinking of DNA-Protein Complexes via SAzidoadenine
Filter-Binding Assays
The Gel Shift Assay for the Analysis of DNA-Protein Interactions
Improved Plasmid Vectors for the Analysis of Protein-Induced DNA Bending
Determination of Sequence Preferences of DNA Binding Proteins Using Pooled Solid-Phase Sequencing of Low Degeneracy Oligonucleotide Mixtures
Analysis of DNA-Protein Interactions by Intrinsic Fluorescence
A Competition Assay for DNA Binding Using the Fluorescent Probe ANS
Circular Dichroism for the Analysis of Protein-DNA Interactions
Electron Microscopy of Protein-Nucleic Acid Complexes: Uniform Spreading and Determination of Helix Handedness
Reconstitution of Protein-DNA Complexes for Crystallization
Assay of Restriction Endonucleases Using Oligonucleotides
Assays for Restriction Endonucleases Using Plasmid Substrates
Assays for Transcription Factor Activity
An Assay for In Vitro Recombination Between Duplex DNA Molecules