Walker J.M. (ed.) Nucleic Acids

Humana Press, 1984. - 366 p. ISBN: 978-0-89603-064-0 (Print) 978-1-59259-489-4 (Online)
Series: Methods in Molecular Biology, №2The Burton Assay for DNA
DABA Fluorescence Assay for Submicrogram Amounts of DNA
Preparation of RNase-Free DNase by Alkylation
The Isolation of Satellite DNA by Density Gradient Centrifugation
The Isolation of High Molecular Weight Eukaryotic DNA
Preparation of Lyophilized Cells to Preserve Enzyme Activities and High Molecular Weight Nucleic Acids
Agarose Gel Electrophoresis of DNA
Autoradiography of Gels Containing 32P
Detection of Specific DNA Sequences—The Southern Transfer
The Extraction and Isolation of DNA from Gels
One-Dimensional Electrophoresis of Nucleic Acids in Agarose Using Denaturation with Formaldehyde and Identification of 3H-Labeled RNA by Fluorography
Gel Electrophoresis of RNA in Agarose and Polyacrylamide Under Nondenaturing Conditions
The Extraction of Total RNA by the Detergent and Phenol Method
RNA Extraction by the Proteinase K Method
RNA Extraction by the Guanidine Thiocyanate Procedure
The Purification of Poly(A)-Containing RNA by Affinity Chromatography
Messenger RNA Fractionation on Neutral Sucrose Gradients
The Estimation of mRNA Content by Poly(U) Hybridization
DNA Directed in Vitro Protein Synthesis with Escherichia coli S-30 Extracts
In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System
In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System
Immunoprecipitation of in Vitro Translation Products with Protein A Bound to Sepharose
In Vitro Continuation of RNA Synthesis Initiated in Vivo
Synthesis of Double-Stranded Complementary DNA from Poly(A)+mRNA
Plasmid DNA Isolation by the Cleared Lysate Method
Plasmid DNA Isolation (Sheared Lysate Method)
Small-Scale Plasmid DNA Preparation
Preparation of Chromosomal DNA from E. coli
Preparation and Assay of Phage Lambda
Preparation of Phage Lambda DNA
The Use of Restriction Endonucleases
Ligation of DNA with T4 DNA Ligase
The Use of Alkaline Phosphatase to Prevent Vector Regeneration
Bacterial Transformation
Bacterial Transformation (Kushner Method)
In Vitro Packaging of DNA
Yeast Transformation
Radiolabeling of DNA by Nick Translation
Radiolabeling of DNA Using Polynucleotide Kinase
Radiolabeling of DNA with 3′ Terminal Transferase
Radiolabeling of DNA with the Klenow Fragment of DNA Polymerase
Identification of Recombinant Plasmids by In Situ Colony Hybridization
Identification of Recombinant Phages by Plaque Hybridization
Plasmid Screening Using Single Colony Lysates
Immunological Detection of Gene Expression in Recombinant Clones
The Isolation of Minicells
The Isolation of Maxicells
The Identification of Gene Products in Minicells and Maxicells
Molecular Cloning in Bacteriophage Lambda and in Cosmids
DNA Transformation of Mammalian Cells
Chemical Cleavage (Maxam and Gilbert) Method for DNA Sequence Determination
Gel Electrophoretic Analysis of DNA Sequencing Products
DNA Sequence Determination Using Dideoxy Analogs