Didenko V.V. (ed.) In Situ Detection of DNA Damage

Humana Press, 2002. - 279 p. - Methods in Molecular Biology, Vol.
203. - Detection and analysis of DNA damage is critically important in the study of the widest variety of biological phenomena, ranging from apoptosis and aging to heart diseases and anticancer drug development. In In Situ Detection of DNA Damage: Methods and Protocols, Vladimir Didenko and a panel of experts describe all the major in situ techniques for studying DNA damage and apoptosis, and show how approaches originally designed to label apoptotic cells can be used for DNA damage analysis (and vice versa). The readily reproducible techniques presented here include all those designed to detect specific single- and double-stranded DNA breaks in tissue sections while using polymerases, ligases, nucleases, and kinases. In-depth protocols for single-cell electrophoresis (comet assay) are presented, as well as those for the detection of indirect markers of DNA damage and modified bases. Technical reviews discuss specificity, sensitivity, advantages, and limitations of the techniques described, and compare them with alternative approaches.
State-of-the-art and highly practical, In Situ Detection of DNA Damage: Methods and Protocols presents for the first time a complete set of enzymatic methods for determining DNA breaks, and will greatly facilitate the work of all those studying not only the in situ detection of DNA damage but also the labeling of apoptotic cells.Color Plates
Labeling DNA Breaks Using Terminal Transferase (TUNEL Assay)
Labeling DNA Damage with Terminal Transferase: Applicability, Specificity, and Limitations
TUNEL Assay: An Overview of Techniques
Electron Microscopic Detection of DNA Damage Labeled by TUNEL
Quantitative Differentiation of Both Free 3' OH and 5' OH DNA Ends Using Terminal Transferase-Based Labeling Combined with Transmission Electron Microscopy
Determination of Three-Dimensional Distribution of Apoptotic DNA Damage by Combination of TUNEL and Quick-Freezing and Deep-Etching Techniques
In Situ Detection of DNA Strand Breaks in Analysis of Apoptosis by Flow- and Laser-Scanning Cytometry
Labeling DNA Breaks Using DNA Polymerase I or its Klenow Fragment
DNA Damage Detection Using DNA Polymerase I or its Klenow Fragment: Applicability, Specificity, Limitations
Labeling DNA Breaks In Situ by Klenow Enzyme
In Situ Nick Translation at the Electron Microscopic Level
Labeling DNA Breaks Using Ligase
In Situ DNA Ligation as a Method for Labeling Apoptotic Cells in Tissue Sections: An Overview
Detection of Specific Double-Strand DNA Breaks and Apoptosis In Situ Using T4 DNA Ligase
In Situ Detection of Double-Strand DNA Breaks with Terminal 5'OH Groups
Detection of DNA Breaks in Agarose Trapped Cells: Comet Assay and Related Techniques
The Comet Assay: Principles, Applications, and Limitations
The Comet Assay: An Overview of Techniques
Ultrasensitive Detection of DNA Damage by the Combination of the Comet and TUNEL Assays
Application of FISH to Detect DNA Damage: DNA Breakage Detection-FISH (DBD-FISH)
Detection of Modified Bases and AP Sites in DNA
Simultaneous In Situ Detection of DNA Fragmentation and RNA/DNA Oxidative Damage Using TUNEL Assay and Immunohistochemical Labeling for 8-Hydroxy-2'-Deoxyguanosine (8-OHdG)
The In Situ Detection of Apurinic/Apyrimidinic Sites and DNA Breaks Bearing Extension Blocking Termini
Indirect and General Markers of DNA Damage
Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage
Ultrasound Imaging of Apoptosis: DNA-Damage Effects Visualized
p53 Induction as an Indicator of DNA Damage
Detection of Caspases Activation In Situ by Fluorochrome-Labeled Inhibitors of Caspases (FLICA)